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No. Title Authors Journal
170 Sentinel cells programmed to respond to environmental DNA including human sequences Xuefei Angelina Nou, Christopher A. Voigt Nature chemical biology (2024) 20(): 211-220
Abstract
Monitoring environmental DNA can track the presence of organisms, from viruses to animals, but requires continuous sampling of transient sequences from a complex milieu. Here we designed living sentinels using Bacillus subtilis to report the uptake of a DNA sequence after matching it to a preencoded target. Overexpression of ComK increased DNA uptake 3,000-fold, allowing for femtomolar detection in samples dominated by background DNA. This capability was demonstrated using human sequences containing single-nucleotide polymorphisms (SNPs) associated with facial features. Sequences were recorded with high efficiency and were protected from nucleases for weeks. The SNP could be determined by sequencing or in vivo using CRISPR interference to turn on reporter expression in response to a specific base. Multiple SNPs were recorded by one cell or through a consortium in which each member recorded a different sequence. Sentinel cells could surveil for specific sequences over long periods of time for applications spanning forensics, ecology and epidemiology.

Presenter : Seongmin Kim
Date : 2024.10.24.THU 18:00

김성민 학생의 주도하에 2024년 Nat Chem Biol. 에 Publish 된 Sentinel cell 에 대해 이야기하는 시간을 가졌습니다.