| No. | Title | Authors | Journal |
|---|---|---|---|
| 19 | Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites. | Park J, Bae S, Kim JS. | Bioinformatics. (2015) 31(24): 4014-6 |
Abstract
We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions.
/ PMID : 26358729
/ presentor : Seongmin Cheon
/ date : 2016.03.09
We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions.
/ PMID : 26358729
/ presentor : Seongmin Cheon
/ date : 2016.03.09