| No. | Title | Authors | Journal |
|---|---|---|---|
| 93 | MiR144-3p is associated with pathologic inflammation in the patients infected with Mycobacteroides abscessus | 60. Kim HJ, Kim IS, Lee SG, Kim YJ, Silwal P, Kim JY, Kim JK, Seo W, Chung C, Cho HK, Huh HJ, Shim SC, Park C, Jhun BW, Jo EK | Experimental & Molecular Medicine (2021) 53(): 136-149 |
Abstract
The infection with rapid growing non-tuberculous mycobacteria is emerging as global health issue; however, key host factors remain elusive. Here we investigated the characteristic immune profiles in peripheral blood mononuclear cells (PBMCs) from the patients infected with Mycobacteroides abscessus subsp. abscessus (Mabc) and M. abscessus subsp. massiliense (Mmass). Using an integrated analysis of global mRNA and microRNA expression profiles, we found that several inflammatory cytokines/chemokines [interleukin (IL)-1β, IL-6, C-X-C motif chemokine ligand 2, and C-C motif chemokine ligand 2] and miR-144-3p were significantly upregulated in PBMCs from patients, compared with those from healthy controls (HC). Notably, there was a strong correlation in the expression levels between miR-144-3p and proinflammatory cytokines/chemokines. Similarly, upregulated expression of miR-144-3p and proinflammatory cytokines/chemokines was found in macrophages and the lungs from mice after infection with Mabc and Mmass. We showed that the expression of negative regulators of inflammation (SARM1 and TNIP3) were significantly down-regulated in PBMCs from the patients, although they were not putative targets of miR-144-3p. Furthermore, overexpression of miR-144-3p led to a marked increase of proinflammatory cytokines/chemokines and promoted bacterial growth in macrophages. Together, our results highlight the implication of miR144-3p linking to pathological inflammation during M. abscessus infection.
The infection with rapid growing non-tuberculous mycobacteria is emerging as global health issue; however, key host factors remain elusive. Here we investigated the characteristic immune profiles in peripheral blood mononuclear cells (PBMCs) from the patients infected with Mycobacteroides abscessus subsp. abscessus (Mabc) and M. abscessus subsp. massiliense (Mmass). Using an integrated analysis of global mRNA and microRNA expression profiles, we found that several inflammatory cytokines/chemokines [interleukin (IL)-1β, IL-6, C-X-C motif chemokine ligand 2, and C-C motif chemokine ligand 2] and miR-144-3p were significantly upregulated in PBMCs from patients, compared with those from healthy controls (HC). Notably, there was a strong correlation in the expression levels between miR-144-3p and proinflammatory cytokines/chemokines. Similarly, upregulated expression of miR-144-3p and proinflammatory cytokines/chemokines was found in macrophages and the lungs from mice after infection with Mabc and Mmass. We showed that the expression of negative regulators of inflammation (SARM1 and TNIP3) were significantly down-regulated in PBMCs from the patients, although they were not putative targets of miR-144-3p. Furthermore, overexpression of miR-144-3p led to a marked increase of proinflammatory cytokines/chemokines and promoted bacterial growth in macrophages. Together, our results highlight the implication of miR144-3p linking to pathological inflammation during M. abscessus infection.