Warning: count(): Parameter must be an array or an object that implements Countable in /var3/home/compsysbio/web/simboardview.php on line 852

Warning: count(): Parameter must be an array or an object that implements Countable in /var3/home/compsysbio/web/simboardview.php on line 853
CSB
sign in board
No. Title Date Creator
3 ‘Proteomics’ 저널에 논문 게재 2016-06-01 20:13:58 csb_hhh (Manager)
이전 Marine genomics에 이어서 조지훈 학생의 연구주제였던 ‘State-of-the-art housekeeping proteins for quantitative western blotting: Revisiting the first draft of the human proteome’ 논문이 2016년 4월 국제학술지 ‘Proteomics’저널에 출판 승인 되었습니다.


이 논문 역시 6월 중 온라인 출판 될 예정입니다.


모두 수고하셨습니다!




논문명 : State-of-the-art housekeeping proteins for quantitative western blotting: Revisiting the first draft of the human proteome



저자 : Hyun-Gwan Lee*, Jihoon Jo∗, Hyun-Hee Hong, Kee K. Kim, Joong-Ki Park, Sung-Jin Cho and Chungoo Park




영문초록 : Western blotting (WB) analysis is the most popular and widely used methodology for protein
detection and characterization over recent decades. In accordance with the advancement of
the technologies for the acquisition of WB signals, a quantitative value is used to present
the abundance of target proteins in a complex sample, thereby requiring the use of specific
proteins as internal references that represent total proteins. Heretofore, proteins encoded by
housekeeping genes such as GAPDH, -tubulin and -actin have been commonly used as
loading controls without any hesitation because their mRNA expression levels tend to be
high and constant in many different cells and tissues. Experimentally, however, some of the
housekeeping reference proteins are often displayed with inconsistent expression levels in
both homogeneous and heterogeneous tissues, and, in terms of mRNA levels, they have a
weak correlation to the abundance of proteins. To estimate accurate, reliable, and reproducible
protein quantifications, it is crucial to define appropriate reference controls. For this paper, we
explored the recently released large-scale, human proteomic database ProteomicsDB including
16 857 liquid chromatography tandem-mass-spectrometry data from 27 human tissues, and
suggest 20 ubiquitously- and constitutively-expressed, putative internal-reference controls for
the quantification of differential protein expressions. Intriguingly, the most commonly used,
known housekeeping genes were entirely excluded in our newly defined candidates. Although
the applications of the candidates under many different biological conditions and in other
organisms are yet to be empirically verified, we propose reliable, potential loading controls for
a WB analysis in this paper.